Regulatory
Part:BBa_K113009
Designed by: Zheng Wei, Wang Jinyu Group: iGEM08_HKUSTers (2008-10-26)
pBad/araC
It is the same as BBa_I0050. But the part seems not working so we make a new one, which the template is from iHKU team.
pBad is a promoter that is tightly controlled by an inducer (L-arabinose) and a repressor (araC). We use it to tightly control the expression of T7 polymerase as in our project the amount of T7 polymerase is essential. (BBa_K113007)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
[edit]
Categories
Parameters
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/negative
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/negative
negative_regulators | 1 |
positive_regulators |